Thermo Fisher Scientific TDG Polyclonal Antibody
TDG 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ChIP assay에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS 기반 완충액에 보관. 인간 TDG 단백질(aa 1-410) 유래 면역원 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| ChIP assay (ChIP) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 1–410 of Human TDG (UniProt ID: Q13569-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546616 |
Product Specific Information
- Recommended positive controls: HeLa, Jurkat, Raji, NCI-H929, TDG-transfected 293T
- Predicted reactivity: Human (99%), Mouse (88%), Rat (87%), Bovine (91%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family.
Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine.
With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine.
This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
This gene may have a pseudogene in the p arm of chromosome 12.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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