Thermo Fisher Scientific CISD2 Monoclonal Antibody (OTI4E8), TrueMAB
CISD2 단백질을 인식하는 mouse monoclonal antibody로 Western blot과 IHC에 적합. Human, Mouse, Rat 반응성. Lyophilized 형태로 제공되며 PBS buffer, trehalose 포함. 연구용으로 Wolfram syndrome 2 관련 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E8 |
| Immunogen | Full length human recombinant protein of human CISD2 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Defects in the CISD2 (CDGSH iron sulfur domain 2) gene cause the neurodegenerative disorder Wolfram syndrome 2.
CISD2 is a zinc finger protein localized to the endoplasmic reticulum and mitochondria, binding an iron/sulfur cluster.
It interacts with Bcl-2 and regulates BIK-initiated autophagy.
CISD2 deficiency in mice leads to mitochondrial breakdown, autophagic cell death, and premature aging phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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