Thermo Fisher Scientific Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Dog, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

34H14L15

Immunogen

A peptide corresponding to amino acids 224-237 of P56856. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Claudin 18,uniProtId:P56856-1,ncbiNodeId:9606,antigenRange:224-237,antigenLength:261,antigenImageFileName:700178_Claudin_18_P56856-1_Rabbit.svg,antigenImageFileNamePDP:700178_Claudin_18_P56856-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532290

Product Specific Information

This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.The immunogenic sequence for this antibody is GFKASTGFGSNTKNH, and the antibody is predicted to react with chimpanzee, equine, rat, and Rhesus monkey based on sequence identity and similarity.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.