Thermo Fisher Scientific CD148 Monoclonal Antibody (1D2C11)
CD148 단백질을 인식하는 Mouse IgG2b 단일클론 항체로 Western blot, Flow cytometry, ELISA에 사용 가능. 단백질 G으로 정제된 액상 형태이며 1 mg/mL 농도. 인간 CD148 단백질 연구용으로 적합하며 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D2C11 |
| Immunogen | Purified recombinant fragment of human CD148 (AA: extra 36–210) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090788 |
Target Information
CD148 is a highly glycosylated transmembrane protein (~250 kDa) containing eight fibronectin type III domains in the extracellular region and one tyrosine phosphatase domain in the cytoplasmic region. It is expressed broadly on fibroblasts, epithelial cells, lymphocytes (B, T, NK cells), and myeloid cells (monocytes, granulocytes). CD148 can dephosphorylate proteins such as LAT and PLC-gamma and is involved in the downregulation of T cell activation. Conversely, CD148 expression can be upregulated following T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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