Thermo Fisher Scientific OCT4 Polyclonal Antibody
OCT4 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB 및 IHC에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제. 줄기세포 연구 및 iPS 세포 생성 관련 연구에 적합.
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Thermo Fisher Scientific OCT4 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | 5 µg/mL | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 14 amino acid peptide near the carboxy terminus of human POU5F1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154512 |
Product Specific Information
- Suggested positive control: Mouse liver tissue lysate
- PA5-20887 can be used with blocking peptide PEP-1001
Target Information
POU5F1, also known as Oct-4, is a maternally expressed octamer-binding protein and the first transcription factor described for early developmental stages.
Its role in embryonic development suggests utility in stem cell creation and cell replacement therapies for degenerative diseases.
Induced pluripotent stem (iPS) cells can be generated by expressing POU5F1 with Sox2, Klf4, Lin28, and c-Myc in mouse fibroblasts.
Recent experiments show iPS cells can be produced using expression plasmids without viral introduction, improving safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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