Thermo Fisher Scientific Histone H4 Polyclonal Antibody
Rabbit polyclonal antibody against human Histone H4, validated for WB, ICC/IF, and ChIP. Affinity purified, unconjugated, and supplied in liquid form. Suitable for research use only; predicted cross-reactivity with mouse, monkey, and dog.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | - |
| ChIP assay (ChIP) | 1–2 µg/1×10⁶ cells | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Protein corresponding to Human HIST1H4A (aa 1–126) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 30% glycerol, 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608369 |
Product Specific Information
These rabbit polyclonal antibodies are developed by immunizing animals with specific proteins or peptides. The antibodies are affinity purified from rabbit sera and tested for functionality in various research applications.
This product is for Research Use Only and is non-hazardous and non-infectious.
Predicted to react with Mouse, Monkey, and Dog.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped into nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
The histone H4 gene is intronless, encodes a member of the histone H4 family, and is located in the large histone gene cluster on chromosome 6. Transcripts lack polyA tails but contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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