Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI3B2), TrueMAB
EpCAM 단백질을 인식하는 Mouse IgG2b 단클론 항체로, Western blot 및 Immunocytochemistry에 적합. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제. 액상 형태로 -20°C 보관하며 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:4,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B2 |
| Immunogen | Full length human recombinant protein of human EPCAM produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues with a molecular weight of approximately 40 kDa. It functions as a Ca²⁺-independent homotypic adhesion molecule, influencing cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial cell differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for tumors of epithelial lineage.
Ep-CAM is found on the basolateral cell surface of most simple epithelia and various carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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