Thermo Fisher Scientific CD21/CD35 Monoclonal Antibody (eBio4E3 (4E3)), FITC, eBioscience
마우스 CD21/CD35 인식 단클론 항체로, FITC 형광 표지되어 Flow Cytometry 및 IHC(F) 분석에 적합합니다. B세포 표면 단백질 분석에 사용되며, 0.5 µg/test 농도로 최적화되어 있습니다. 4°C 암소 보관, 동결 금지.
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카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD21/CD35 Monoclonal Antibody (eBio4E3 (4E3)), FITC, eBioscience
Applications
Immunohistochemistry (Frozen) (IHC (F))
- Tested Dilution: -
- Publications: View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
- Publications: View 8 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio4E3 (4E3) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_464975 |
Available Formats
Product Specific Information
The monoclonal antibody eBio4E3 reacts with an epitope shared by mouse CD21 (CR2) and CD35 (CR1).
CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, resulting in surface proteins of 145 and 190 kDa, respectively.
They are expressed on mature B cells, but not on thymocytes, peripheral T cells, erythrocytes, or platelets. Expression on macrophages is minimal.
Functional Roles:
- CD21 acts as a receptor for complement component C3d and Epstein-Barr virus (EBV).
- In association with CD19 and CD81, CD21 participates in B-cell activation.
- Cr2-deficient mice show impaired inflammatory and humoral immune responses.
Clone Characteristics:
- eBio4E3 and eBio8D9 do not cross-block each other, indicating different epitope binding.
- Reported application: Flow cytometric analysis of mouse splenocytes.
- Recommended usage: ≤0.5 µg/test (100 µL final volume; 10⁵–10⁸ cells/test).
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD21 (Complement Receptor 2, CR2)
Binds C3 complement fragments and plays a key role in:
- Uptake and retention of immune complexes
- Survival of memory B cells
- Maintenance of humoral immune responses
Also serves as a receptor for Epstein-Barr virus and contributes to prion targeting to follicular dendritic cells.
CD35 (Complement Receptor 1, CR1)
Encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells.
Acts as a receptor for complement components C3b and C4b, regulating complement cascade activity.
Variations in CD35 form the basis of the Knops blood group system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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