Thermo Fisher Scientific DNER Polyclonal Antibody, FITC
DNER 단백질을 인식하는 FITC 결합 토끼 폴리클로날 항체. Western blot, ELISA, IP에 적합. 인간, 마우스, 랫트 반응성. 액상 형태로 제공되며 친화 크로마토그래피로 정제됨. -20°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 700–737 on human Delta and Notch-like Epidermal growth factor-related receptor protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
DNER (Delta-notch-like EGF-related receptor), also known as Delta-notch-like EGF repeat-containing transmembrane protein, is a neuron-specific, atypical Notch ligand expressed in dendrites and cell bodies of neurons throughout the central nervous system.
DNER contains ten extracellular EGF-like domains that are highly homologous to those of the Notch ligand, Delta.
In the cerebellum, DNER is predominantly expressed in Purkinje cells and mediates neuron-glia interaction during astrocytogenesis through direct interaction with Notch 1 at Purkinje cell/Bergmann glia contacts.
This interaction activates a Deltex-dependent Notch signaling pathway in Bergmann glia and may regulate Bergmann glial morphogenesis.
DNER is crucial for the functional and morphological maturation of Bergmann glia. DNER-knockout mice show motor discoordination and cerebellar morphogenesis retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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