Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Monoclonal Antibody (MILAN8R), PE-eFluor 610, eBioscience
인간 및 마우스 ERK1/2 인산화형(T202/Y204)을 인식하는 MILAN8R 단일클론 항체로, PE-eFluor 610 형광체 결합. 세포내 염색 및 유세포분석에 최적화되어 있으며, 높은 특이성과 안정성을 제공. 4°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Monoclonal Antibody (MILAN8R), PE-eFluor 610
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MILAN8R |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574675 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- PE
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
This MILAN8R monoclonal antibody recognizes human and mouse ERK1/2 (p44/p42, MAPK3/1) phosphorylated on T202/Y204. ERK1/2 are key MAPKs involved in proliferation, differentiation, motility, and survival. Activation occurs via MEK1/2-mediated phosphorylation, and downstream targets include p90RSK and Elk-1. ERK signaling dysregulation is common in cancer.
Specificity confirmed by ELISA, flow cytometry, and western blotting.
Reported applications: intracellular staining followed by flow cytometric analysis.
Tested applications: pre-titrated for intracellular staining of human PBMCs at 5 µL/test (0.5 µg). A test corresponds to 100 µL final volume, with 10⁵–10⁸ cells/test.
Staining Protocol
Use Protocol C: Two-step (Fixation/Methanol).
Protocols A and B (cytoplasmic/nuclear) are not suitable.
Detailed protocols available in BestProtocols® online resource: Staining Intracellular Antigens for Flow Cytometry
Fluorophore Information
PE-eFluor® 610 excitation: 488–561 nm; emission: 607 nm.
Recommended filter: 610/20 band pass (PE-Texas Red® equivalent).
Sensitive to photo-induced oxidation — protect from light.
Fixation
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Store up to 3 days at 4°C in dark with minimal brightness loss.
Clone-specific performance should be validated empirically.
Laser Compatibility
- Excitation: 488–561 nm
- Emission: 607 nm
- Compatible lasers: Blue, Green, Yellow-Green
Filtration
Post-manufacturing filtered (0.2 µm).
Target Information
ERK1 and ERK2 regulate meiosis, mitosis, and postmitotic functions. Activation occurs via Ras-Raf-MEK-ERK signaling cascade upon growth factor stimulation. Activated ERKs phosphorylate cytoplasmic targets or nuclear transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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