Thermo Fisher Scientific FEN1 Recombinant Rabbit Monoclonal Antibody (ARC1376)
FEN1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA 등에 사용 가능. 인간 및 생쥐 시료에 반응하며, HEK293 세포에서 발현된 IgG 형식. DNA 복제 및 수선 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1376 |
| Immunogen | Synthetic peptide corresponding to amino acids 250–350 of human FEN-1 (P39748) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849643 |
Product Specific Information
Immunogen sequence:
IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS L
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein functioning as an endonuclease that cleaves branched DNA structures containing single-stranded 5′ flaps, or as an exonuclease on DNA nicks and gaps. Its activity is stimulated by association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 plays critical roles in DNA replication and repair and is essential in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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