Thermo Fisher Scientific CD25 Monoclonal Antibody (BC96), Alexa Fluor 647, eBioscience
인간 CD25(IL-2Rα) 인식 단일클론 항체로, Alexa Fluor 647로 표지되어 유세포분석(Flow Cytometry)에 최적화됨. BC96 클론 기반 IgG1, κ형 항체로 고품질 친화 크로마토그래피 정제. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 23 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, κ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BC96 |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744736 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615 / 737 / 805
- Brilliant Violet 421 / 711
- eFluor 450
- FITC
- NovaFluor Red 685 / 700
- NovaFluor Yellow 700
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
Description:
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone but forms a high-affinity receptor complex with CD122 (IL-2Rβ) and CD132 (common γ chain). CD25 plays a role in lymphocyte differentiation, activation, and proliferation.
Applications Reported:
This BC96 antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (in 100 µL final volume). Optimal cell number: 10⁵–10⁸ cells/test.
Fluorescence Properties:
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
Target Information
CD25 (IL2 receptor alpha chain / IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The receptor complex includes IL2RA, IL2RB, and IL2RG, forming a high-affinity IL-2 receptor. Homodimeric IL2RA forms a low-affinity receptor, while homodimeric IL2RB forms a medium-affinity receptor. IL2 is primarily produced by mature T cells and functions as a growth, differentiation, and apoptosis-regulating factor. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency and immune dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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