Thermo Fisher Scientific FITC Monoclonal Antibody (LO-FLUO-1), FITC

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

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View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

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Product Specifications

Published species

Chemical

Host/Isotype

Rat / IgM

Class

Monoclonal

Type

Antibody

Clone

LO-FLUO-1

Immunogen

FITC-labeled mouse IgG1 kappa

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Storage conditions

-20° C, store in dark

Shipping conditions

Dry ice

RRID

AB_2536400

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.