Thermo Fisher Scientific Mouse IL-4 Recombinant Protein
Recombinant mouse IL-4 protein expressed in E. coli, >96% purity. Suitable for WB, IHC, ELISA, and functional assays. Promotes HT-2 cell proliferation with ED50 ≤ 2 ng/mL. Lyophilized form, stable under proper storage conditions for research use only.
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- Protein
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- pk
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Mouse |
| Expression System | E. coli |
| Amino Acid Sequence | MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S |
| Molecular Weight | 13.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >96% by SDS-PAGE and RP-HPLC |
| Activity | ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of mouse HT-2 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Reconstitution:
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute lyophilized mIL-4 in sterile 10 mM HAc at not less than 100 µg/mL, then dilute further as needed. For long-term storage, add a carrier protein (e.g., 0.1% HSA or BSA).
Storage:
Stable at room temperature for 3 weeks; store desiccated below -18°C. After reconstitution, stable at 4°C for up to 1 week; store below -18°C for long-term use. Avoid repeated freeze/thaw cycles.
Target Information
Interleukin-4 (IL-4) is a pleiotropic immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor. It has broad biological functions and shares overlapping roles with IL-13 due to receptor binding similarities. STAT6 mediates IL-4 signal transduction. The IL-4 gene is part of a cytokine cluster on chromosome 5q, closely located to IL-13 and IL-5, coordinated by long-range regulatory elements. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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