Thermo Fisher Scientific Involucrin Polyclonal Antibody
Thermo Fisher Scientific의 Involucrin Polyclonal Antibody는 사람 Involucrin 단백질을 인식하는 토끼 IgG 기반 항체로, WB, IHC, ICC/IF에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 50–142 of Human Involucrin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.72 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548056 |
Product Specific Information
- Recommended positive control: Raji
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Involucrin is a component of the keratinocyte crosslinked envelope, found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. It is expressed in stratified squamous epithelia, including the cornea. In normal epidermis, it appears in the upper spinous layers, and in keratinocyte cultures, it is expressed by cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and reduced in severe dysplasias of the larynx and cervix. The gene is mapped to chromosome 1q21 among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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