Thermo Fisher Scientific HGK Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 383 and 437 of Human MAP4K4 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HGK,uniProtId:O95819-1,ncbiNodeId:9606,antigenRange:383-437,antigenLength:1239,antigenImageFileName:PA5-30579_HGK_O95819-1_Rabbit.svg,antigenImageFileNamePDP:PA5-30579_HGK_O95819-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548053

Product Specific Information

Recommended positive controls: 293T, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (100%), Rat (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP4K4 (HGK) is a serine/threonine kinase which plays an important role in stress-activated MAPK core signaling pathways, the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.