Thermo Fisher Scientific TPL2 Polyclonal Antibody
TPL2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 생쥐, 랫드에서 반응하며, 고순도 Affinity chromatography 정제. MAP3K8/COT 단백질의 내인성 수준 검출에 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP3K8 (Accession P41279), corresponding to amino acid residues T264–G314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2900177 |
Product Specific Information
Antibody detects endogenous levels of total MAP3K8/COT.
Target Information
MAP3K8 (COT) is a serine/threonine kinase that activates both MAP and JNK kinase pathways and plays a role in the cell cycle.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or ERK, MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKKK/MEKK phosphorylates and activates MAPK kinase/MEK, which in turn activates MAPK.
These kinases are highly conserved among species such as yeast, Drosophila, and mammals. MEKK8 activates NF-kappa-B1 by stimulating proteasome-mediated proteolysis of NF-kappa-B1/p105.
This cytoplasmic protein is expressed in several normal tissues and tumor-derived cell lines. The 58 kDa form is activated during S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation mainly on Ser residues, while the shorter form on both Ser and Thr residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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