Thermo Fisher Scientific IRS1 Monoclonal Antibody (10I3)
Thermo Fisher Scientific의 IRS1 단클론 항체(10I3)는 인간 IRS1 단백질을 인식하며 Western blot, IHC, ICC, 유세포 분석에 적합합니다. 고순도의 친화 크로마토그래피 정제 제품으로, 동결건조 형태로 제공되어 안정적 장기 보관이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10I3 |
| Immunogen | E.coli-derived human IRS1 recombinant protein (Position: S1041–Q1242). Human IRS1 shares 78% and 80% amino acid sequence identity with mouse and rat IRS1, respectively. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2884056 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4). It undergoes phosphorylation on serine, threonine, and tyrosine residues across various tissues. Casein kinase II mediates part of the insulin-stimulated phosphorylation of IRS-1, identifying Thr 502 and Ser 99 as key phosphorylation sites. IRS-1 includes three putative 14-3-3 binding sites (Ser 270, Ser 374, Ser 641), with the Ser 270 motif located in the phosphotyrosine binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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