Thermo Fisher Scientific Endostatin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to residues R(1718) R L M E S Y C E T W R T E(1731) of Mouse Endostatin and R(1699) R L T E S Y C E T W R T E(1712) of Human Endostatin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Endostatin,uniProtId:P39060-3,ncbiNodeId:9606,antigenRange:1699-1712,antigenLength:1754,antigenImageFileName:PA1-601_Endostatin_P39060-3_Rabbit.svg,antigenImageFileNamePDP:PA1-601_Endostatin_P39060-3_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Endostatin,uniProtId:P39061-3,ncbiNodeId:10090,antigenRange:1718-1731,antigenLength:1774,antigenImageFileName:PA1-601_Endostatin_P39061-3_Rabbit.svg,antigenImageFileNamePDP:PA1-601_Endostatin_P39061-3_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2245014

Product Specific Information

PA1-601 detects recombinant human and mouse endostatin.

PA1-601 has been successfully used in Western blot, IF/ICC and IHC (P) procedures. By Western blot, this antibody detects a 20 kDa protein representing recombinant human endostatin.

PA1-601 immunizing peptides are two peptides derived from mouse and human endostatin protein corresponding to amino acid residues 129-142 of endostatin.

PA1-601 can be used with blocking peptide PEP-112.

Target Information

Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer`s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.