Thermo Fisher Scientific Pan SHANK Monoclonal Antibody (N23b/49), PerCP
Pan SHANK 단백질을 인식하는 PerCP 결합 단일클론 항체로, Western blot, IHC, ICC/IF, IP, AM 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 단백질 G 정제, 1 mg/mL 농도, 보존제 없음. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N23b/49 |
| Immunogen | Fusion protein corresponding to amino acids 84–309 of rat Shank2 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931894 |
Available Formats
Product Specific Information
- 1 µg/mL of MA5-45440 was sufficient for detection of Shank1–4 in 10 µg of rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 160 kDa.
- Recognizes Shank1, Shank2, and Shank3.
Target Information
Shank proteins are a family of scaffold proteins that interact with diverse membrane and cytoplasmic proteins. They are localized at postsynaptic sites of excitatory synapses and play a role in signal transduction into postsynaptic neurons.
Shank2 is expressed in neurons of the developing retina and may contribute to neuronal differentiation. Disruption of glutamate receptors at the Shank postsynaptic platform has been implicated in synaptic dysfunction and loss associated with Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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