Thermo Fisher Scientific Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (ARC1598)
Phospho-ATF2 (Thr71) 특이적 토끼 단클론 항체로, Western blot과 ELISA에 적합합니다. 재조합 형식으로 높은 특이성과 재현성을 제공합니다. ATF2 인산화 연구에 유용하며, 세포 스트레스 반응 및 MAPK 신호전달 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1598 |
| Immunogen | A synthetic phosphorylated peptide around T71 of human ATF2 (P15336) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.52 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898149 |
Product Specific Information
- Positive Samples: NIH/3T3
- Immunogen sequence: TPTPT
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with various viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulates ATF-2 transcriptional activity through phosphorylation at Thr69 and Thr71.
Both SAPK and p38 MAPK phosphorylate ATF-2 at these sites in vitro and in transfected cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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