Thermo Fisher Scientific ESAM Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (IHC)

5-15 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ESAM Gln30-Ser248 (predicted) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ESAM,uniProtId:Q925F2-1,ncbiNodeId:10090,antigenRange:30-248,antigenLength:394,antigenImageFileName:PA5-48019_ESAM_Q925F2-1_Goat.svg,antigenImageFileNamePDP:PA5-48019_ESAM_Q925F2-1_Goat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606280

Product Specific Information

In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human ESAM is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.