
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
Thermo Fisher의 Goat anti-Mouse IgG (H+L) HRP 결합 2차 항체로, 마우스 IgG 검출에 높은 특이성과 교차 흡착성을 제공합니다. WB, IHC, ELISA 등 다양한 응용에 적합하며, 고감도 신호 증폭을 지원합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:200,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534751 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, rabbit, and rat IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin mouse serum proteins, bovine, goat, human, rabbit, or rat IgG.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of specified targets.
Secondary antibodies offer versatility and increased sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, conjugation) to produce highly specific reagents.
Additional Formats
Available conjugate options include:
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 635, 647, 660, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 647, 680, 800
- AP, Biotin, DSB-X Biotin, FITC, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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