Thermo Fisher Scientific ATP6V1F Monoclonal Antibody (OTI5F10)
ATP6V1F 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 고순도 Affinity Chromatography 정제, 안정적인 액상 형태, -20°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific ATP6V1F Monoclonal Antibody (OTI5F10)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5F10 |
| Immunogen | Full length human recombinant protein of ATP6V1F produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722884 |
Target Information
ATP6V1F encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase-dependent organelle acidification is essential for intracellular processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
V-ATPase consists of two domains:
- V1 domain: cytosolic, contains ATP catalytic site with subunits A, B, G, C, D, E, F, and H
- V0 domain: transmembrane, composed of subunits a, c, c’, c”, and d
This encoded protein corresponds to the V1 domain F subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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