Thermo Fisher Scientific SIRP alpha Recombinant Rabbit Monoclonal Antibody (001)

Applications

• ELISA (ELISA): 1:5,000–1:10,000

Product Specifications

Additional Formats

• APC
• FITC
• PE
• Request custom conjugation

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the best candidates for production.
Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to the large rabbit immune repertoire

This antibody specifically recognizes Rat SIRPA/SIRP alpha/CD172a.

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on myeloid cells such as granulocytes, dendritic cells, macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for activated tyrosine kinases (EGFR, PDGFR, src, insulin receptor) and negatively regulates receptor tyrosine kinase-coupled signaling pathways.
Binding of SIRP alpha to CD47 induces phosphorylation of ITIM motifs, leading to recruitment of SHP-1 and SHP-2 phosphatases.
This interaction regulates processes such as inhibition of macrophage phagocytosis, and activation of T cells and dendritic cells.
SIRP alpha plays roles in physiological and pathological processes, including cancer, where CD47-mediated activation inhibits macrophage-mediated tumor cell destruction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.