Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PE, eBioscience
마우스 CD117(c-Kit) 단백질을 인식하는 2B8 단클론 항체로, PE 형광표지되어 있습니다. 유세포분석(Flow Cytometry)에 적합하며, 0.125 µg/test 농도로 사용됩니다. 조혈전구세포 및 비만세포 연구에 활용됩니다. 4°C 보관, 동결 금지.
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- 12-1171-8x (3개 옵션)
- 판매단위
- pk
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3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PE, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 110 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Fish, Guinea pig, Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465812 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor and stem cell factor receptor. CD117 is a 145-kDa tyrosine kinase receptor expressed on most hematopoietic progenitor cells, mast cells, and a subset of bone marrow cells positive for Sca-1 and Thy1 (low), but negative for lineage markers.
The interaction between c-Kit and steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and plays a key role in mast cell activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometry of mouse bone marrow cells at ≤0.125 µg/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL volume. Optimal titration is recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF, also known as stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
The KIT receptor is expressed in hematopoietic stem cells, germ cells, mast cells, and interstitial cells of Cajal. Upon SCF binding, KIT dimerizes and autophosphorylates tyrosine residues (e.g., Tyr703), leading to activation of downstream signaling pathways including Ras/ERK, PI3K, PLC-γ, and JAK/STAT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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