Thermo Fisher Scientific POLR2A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, H1299, HepG2, Raji
• Predicted reactivity: Mouse (100%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
POLR2A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit.
Pol II is the central component of the basal RNA polymerase II transcription machinery, composed of mobile elements that move relative to each other.
RPB1 contributes to the core element with the central large cleft, the clamp element that opens and closes the cleft, and the jaws that grab the incoming DNA template.
During transcription initiation, a single-stranded DNA template is positioned within the active site cleft of Pol II. A bridging helix from RPB1 crosses the cleft near the catalytic site and promotes translocation of Pol II by acting as a ratchet.
Elongation is influenced by phosphorylation of the C-terminal domain (CTD) of RPB1, which regulates transcription initiation, elongation, termination, and mRNA processing. Gene expression regulation depends on the balance between methylation and acetylation levels of CTD lysines.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.