Thermo Fisher Scientific Biotin Ligase Epitope Tag Polyclonal Antibody
Rabbit polyclonal antibody recognizing the Biotin Ligase Epitope Tag (BAP/BLT). Suitable for Western blot and ELISA at 1:5,000 dilution. Detects biotinylated and non-biotinylated tags. Supplied as liquid, 70 mg/mL, stored at -20 °C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| ELISA | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-BIOTIN Ligase Epitope Tag antibody prepared from rabbit serum immunized with a synthetic peptide (GLNDIFEAQKIEWH; minimal BirA substrate; biotin added at lysine). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 70 mg/mL |
| Storage Buffer | 0.02 M potassium phosphate/whole serum, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20 °C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long-term storage. Avoid repeated freeze/thaw cycles. Centrifuge if product is not clear after standing at room temperature. Stable for several weeks at 4 °C as an undiluted liquid. Dilute only immediately before use.
This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA). Western blot analysis of B. subtilis strains producing BAP-tagged SPoIIIAH (XWB49) or SpoIIQ (XWB46) showed strong and specific reactivity. Minor background bands may be observed. The antisera recognizes the BAP/BLT tag regardless of biotinylation status.
Target Information
The peptide is also known as the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase from E. coli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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