Thermo Fisher Scientific Biotin Ligase Epitope Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

ELISA (ELISA)

1:5,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-BIOTIN Ligase Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine.)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

70 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA). Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).

Target Information

The peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.