Thermo Fisher Scientific WTAP Monoclonal Antibody (OTI6H3)
WTAP 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot 및 IHC(Paraffin) 실험에 적합합니다. 인간 시료 반응성이 있으며, 고순도 친화 크로마토그래피 정제 제품입니다. 안정적인 액상 형태로 -20°C 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6H3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–106 of WTAP produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725679 |
Target Information
Wilms’ tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and occurs in both sporadic and inherited forms.
The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a motif common to the Egr family of Zn²⁺ finger transcriptional activators, and functions as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and is involved in regulating mRNA splicing. WTAP localizes to nuclear speckles and regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and reduces WT1 DNA-binding ability. Two isoforms of WTAP exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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