Thermo Fisher Scientific UBE2L3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_0012432841) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UBE2L3,uniProtId:P68036-1,ncbiNodeId:9606,antigenRange:15-154,antigenLength:154,antigenImageFileName:PA5-92154_UBE2L3_P68036-1_Rabbit.svg,antigenImageFileNamePDP:PA5-92154_UBE2L3_P68036-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.27 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806394

Product Specific Information

Immunogen sequence: RKCGMKNFRN IQVDEANLLT WQGLIVPDNP PYDKGAFRIE INFPAEYPFK PPKITFKTKI YHPNIDEKGQ VCLPVISAEN WKPATKTDQV IQSLIALVND PQPEHPLRAD LAEEYSKDRK KFCKNAEEFT KKYGEKRPVD; Positive Samples: U-251MG, HL-60, K-562, HeLa, Mouse testis, Mouse brain, Mouse skeletal muscle; Cellular Location: Cytoplasm, Nucleus

Target Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.