Thermo Fisher Scientific Diphtheria Toxin Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified diphtheria toxoid.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

5.0 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1017028

Product Specific Information

The PA1-73139 antibody reacts with Diphtheria toxin.

PA1-73139 has been successfully used in Immunohistochemical, ELISA, Immunofluorescence and Western blot applications.

The PA1-73139 antibody was raised against purified diphtheria toxoid.

Target Information

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.