Thermo Fisher Scientific AQP2 Polyclonal Antibody
AQP2 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. Human, Mouse, Rat 시료에 반응하며, Protein A로 정제된 고품질 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:250 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human AQP2 (amino acids 171–271) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. Vasopressin-induced cAMP increase results in phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells. Serine-261 has also been identified as a phosphorylation site, with levels of phosphorylated S261 decreasing upon vasopressin treatment, suggesting its role in vasopressin-dependent AQP2 trafficking.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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