Thermo Fisher Scientific Mu-Calpain Polyclonal Antibody
Mu-Calpain 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Human에 반응하고 Mouse, Rat 등에서도 예측 반응성을 보임. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 71–315 of Human Calpain 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.55 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545158 |
Product Specific Information
- Recommended positive controls: A431, Jurkat
- Predicted reactivity: Mouse (87%), Rat (87%), Xenopus laevis (86%), Pig (96%), Chicken (89%), Sheep (95%), Rhesus Monkey (99%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calpains, including Calpain 1 (CAPN1), are calcium-activated neutral proteases functioning as nonlysosomal intracellular cysteine proteases. Mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes are heterodimers with distinct large catalytic subunits and a common small regulatory subunit.
The CAPN1 gene encodes the large subunit of the ubiquitous enzyme calpain 1, with several transcript variants encoding two different isoforms. Diseases associated with CAPN1 include Spastic Paraplegia 76 (Autosomal Recessive) and Spasticity.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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