Thermo Fisher Scientific ATF3 Monoclonal Antibody (7F1B10)
ATF3 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, ICC/IF, Flow cytometry, ELISA에 적합합니다. 정제된 재조합 인간 ATF3(AA 1-181)을 면역원으로 사용하였으며, Protein G로 정제된 액상형 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7F1B10 |
| Immunogen | Purified recombinant fragment of human ATF3 (AA: 1-181) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787259 |
Product Specific Information
MA5-31635 has been tested in indirect ELISA.
Target Information
ATF3 (Activating Transcription Factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kDa.
ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated protein lacking the leucine zipper dimerization motif and does not bind DNA.
In contrast, ATF3 delta Zip stimulates transcription by sequestering inhibitory co-factors away from the promoter. Alternative splicing of the ATF3 gene may be physiologically important in regulating target genes. Three transcript variants encoding two isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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