Thermo Fisher Scientific DKK1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human DKK1 for WB, IHC(P), and ICC/IF applications. High specificity, antigen affinity purified, and supplied as liquid unconjugated form. Suitable for Wnt signaling research.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Horse, Human, Non-human primate, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 16 amino acid peptide from N-terminus of human DKK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550185 |
Product Specific Information
Percent identity with other species by BLAST analysis:
Human, Gorilla, Monkey, Marmoset, Horse, Pig (100%);
Gibbon, Mouse, Rat, Bat, Elephant (94%);
Bovine, Hamster (88%);
Panda, Rabbit (81%).
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
DKK-1 is a member of the DKK protein family, which includes DKK-2, DKK-3, and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that acts as an antagonist for Wnt signaling.
DKK-1 and DKK-4 play key regulatory roles in the Wnt/β-catenin signaling pathway by forming inhibitory complexes with LRP5 and LRP6, essential co-receptors in the pathway.
By inhibiting Wnt/β-catenin signaling, DKK-1 promotes anterior development in vertebrates. Mice lacking DKK-1 expression fail to form heads and die during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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