Thermo Fisher Scientific Human TNF-alpha Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent
• Miscellaneous PubMed (Misc): Refer to 6 publications

Product Specifications

Product Specific Information

The mature, soluble form of human TNF-alpha was expressed in Pichia pastoris (KM71H).
GenBank Accession # P01375, amino acids 77–233.
The expressed protein was N-terminal sequenced and begins with: VRSSSRTPSD.
Purity is >95% by SDS-PAGE analyses.

Activity:
Measured by cytolytic effect on mouse L-929 cells in the presence of Actinomycin D.

Use:
For in vitro applications. Perform dose-response assays to determine optimal concentration for specific experiments.

Reconstitution and Dilution:

• Store lyophilized material at 4°C.
• Reconstitute in 1 mL distilled or deionized water.
• Dilute in buffer containing carrier protein, aliquot, and store at ≤ -70°C.
• Avoid multiple freeze-thaw cycles.
• Suitable buffers: PBS with 1% BSA or neutral media containing <10% FCS.

Target Information

TNF-alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily.
It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
TNF-alpha regulates immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as multimers (2–5 units) but shows a single 17 kDa band under non-reducing SDS-PAGE conditions.
Knockout studies suggest neuroprotective functions and tumor necrosis activity in mice.
TNF-alpha plays key roles in immune responses to bacterial, viral, parasitic, and fungal infections, and is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.