Thermo Fisher Scientific Calprotectin Monoclonal Antibody (MAC387)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:250

-

Flow Cytometry (Flow)

1:50-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Deer, Horse, Cat, Guinea pig, Goat, Human, Non-human primate, Pig, Rabbit, Rat

Published species

Dog, Human, Non-human primate, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MAC387

Immunogen

Human monocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calprotectin,uniProtId:P06702-1,ncbiNodeId:9606,antigenRange:1-114,antigenLength:114,antigenImageFileName:MA5-16808_Calprotectin_P06702-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16808_Calprotectin_P06702-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Calprotectin,uniProtId:P05109-1,ncbiNodeId:9606,antigenRange:1-93,antigenLength:93,antigenImageFileName:MA5-16808_Calprotectin_P05109-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16808_Calprotectin_P05109-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538291

Product Specific Information

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is human spleen. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.

Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain.

Target Information

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.