Thermo Fisher Scientific GKN1 Monoclonal Antibody (759821)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

8-25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

759821

Immunogen

E. coli-derived recombinant mouse Gastrokine 1 Tyr38-Tyr201

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605592

Product Specific Information

This antibody detects mouse Gastrokine 1 in direct ELISAs and mouse and rat Gastrokine 1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Gastrokine 1 and no cross-reactivity with rhBlottin is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

Gastrokine 1, also known as GKN1 and CA11, is a 185 amino acid containing secreted protein belonging to the gastrokine family with a BRICHOS domain and an asn-rich region. GKN1 (also known as an antioncogene) is reported to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. Differential display of normal and cancerous gastric tissue suggested that this protein is predominantly expressed in stomach and the expression is down-regulated to considerable frequency in cancer tissue or gastric cancer cell lines examined. This extra cellular protein is co-secreted with mucins and is a signaling growth factor with mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.