Thermo Fisher Scientific TCF12 Polyclonal Antibody
TCF12 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS buffer에 ProClin 300 포함, 4°C 또는 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:300–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminus of Human TCF12 (UniProt ID: Q99081-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography, Protein A |
| Storage Buffer | PBS, pH 7 |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2866819 |
Product Specific Information
This antibody reacts with Human, Mouse, and Rat (species predicted to react based on 100% sequence homology).
Target Information
TCF12, also known as HTF4, is a basic helix-loop-helix (bHLH) E-protein family member that recognizes the E-box consensus sequence (CANNTG). It is expressed in skeletal muscle, thymus, B- and T-cells, and regulates lineage-specific gene expression through heterodimer formation with other bHLH E-proteins. TCF12, in combination with E2A, is essential for blocking thymocyte proliferation before pre-TCR expression and plays a critical role in T cell differentiation. It is also required for the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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