Thermo Fisher Scientific Biotin Monoclonal Antibody (3E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

1:40,000

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3E6

Immunogen

Biotinylated denatured ovalbumin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.08 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_1070752

Product Specific Information

HYB 212-01-02 detects biotin in samples.

HYB 212-01-02 has been successfully used in ELISA and Western blotting techniques. HYB 212-01-02 reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blotting after SDS-PAGE, HYB 212-01-02 reacts with biotinylated protiens in both reduced and non-reduced forms.

The HYB 212-01-02 immunogen is biotinylated, denatured ovalbumin.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.