Thermo Fisher Scientific N-cadherin Recombinant Rabbit Monoclonal Antibody (020)
Thermo Fisher의 N-cadherin Recombinant Rabbit Monoclonal Antibody (Clone 020)은 인간 N-Cadherin/CD325/CDH2에 특이적입니다. WB, ICC/IF, Flow Cytometry, ELISA에 사용 가능하며, 높은 특이성과 민감도를 제공합니다. 보존제가 없는 액상 형태로 제공되며, 일관된 로트 간 재현성을 보장합니다.
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Thermo Fisher Scientific N-cadherin Recombinant Rabbit Monoclonal Antibody (020)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 020 |
| Immunogen | Recombinant Human N-Cadherin/CD325/CDH2 Protein (Met1–Ala724) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term); -20°C (long term, avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785062 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human N-Cadherin/CD325/CDH2.
Target Information
N-cadherin is a 140 kDa transmembrane protein mediating calcium-dependent intercellular adhesion. It plays a key role in morphogenetic movements during development and regulates cell surface adhesion through homotypic interactions. Its function depends on association with the actin cytoskeleton via catenin proteins, regulated by phosphorylation of beta-catenin.
N-cadherin comprises five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It is essential during gastrulation and for establishing left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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