Thermo Fisher Scientific H4K5ac Recombinant Rabbit Monoclonal Antibody (RM199)
H4K5ac에 특이적인 재조합 토끼 단클론 항체로, 높은 특이성과 일관된 품질을 제공합니다. Western blot, ICC/IF, ELISA, Luminex 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며 Protein A로 정제된 액상 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.05–0.2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM199 |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744938 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction. The histone H4 gene is intronless and encodes a member of the histone H4 family, found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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