Thermo Fisher Scientific Myotilin Polyclonal Antibody
Rabbit polyclonal antibody against human Myotilin. Validated for WB, IHC(P), ICC/IF applications. Recognizes Myotilin (aa 202–482) with predicted cross-reactivity in mouse, rat, pig, and bovine. Supplied as 1 mg/mL liquid, antigen-affinity purified, fo...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | — |
| Immunocytochemistry (ICC/IF) | — | 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 202–482 of human Myotilin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4 °C short term; for long term, store at −20 °C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546535 |
Product Specific Information
- Recommended positive control: A549
- Predicted reactivity: Mouse (93%), Rat (94%), Pig (95%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
Myotilin is a cytoskeletal protein important for maintaining thin filament stability during muscle contraction. It binds F-actin, crosslinks actin filaments, and prevents filament disassembly. Mutations in the Myotilin gene are linked to limb-girdle muscular dystrophy and myofibrillar myopathies. Multiple alternatively spliced variants exist, though not all have been fully characterized.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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