Thermo Fisher Scientific Involucrin Recombinant Rabbit Monoclonal Antibody (8H7B3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8H7B3

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Involucrin (P07476). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Involucrin,uniProtId:P07476-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:585,antigenImageFileName:MA5-42663_Involucrin_P07476-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42663_Involucrin_P07476-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911804

Product Specific Information

Positive test controls include: Rat lung.

Immunogen sequence: KAENPEQQLK QEKTQRDQQL NKQLEEEKKL LDQQLDQELV KRDEQLGMKK EQLLELPEQQ EGHLKHLEQQ EGQLKHPEQQ EGQLELPEQQ EGQLELPEQQ E

Target Information

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.