Thermo Fisher Scientific ARHGEF11 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ARHGEF11(Accession O15085), corresponding to amino acid residues L1432-P1482. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ARHGEF11,uniProtId:O15085-1,ncbiNodeId:9606,antigenRange:1432-1482,antigenLength:1522,antigenImageFileName:PA5-101846_ARHGEF11_O15085-1_Rabbit.svg,antigenImageFileNamePDP:PA5-101846_ARHGEF11_O15085-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2851278

Product Specific Information

Antibody detects endogenous levels of total ARHGEF11.

Target Information

ARHGEF11 is a 1527 amino acid protein with tandem dbl homology and pleckstrin homology domains, PDZ domain, two proline rich sequences and a regulatory G protein-signaling (RGS) sequence. It acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase, but not for RAC1 or CDC42 and acts as GTPase-activating protein (GAP) for GNA12 and GNA13. It forms a complex with G proteins and stimulates neurite through retraction Rho-dependent signals. ARHGEF11 increases glutamate transport 4-fold. It interacts with EAAT4 and modulates its uptake activity and perisynaptic distribution at glutamatergic synapses. It is known to induce the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. It is ubiquitously expressed, in brain, lung, liver, kidney and skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.