Thermo Fisher Scientific LAP2 Monoclonal Antibody (3A3)
인간 LAP2 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, IP에 사용 가능. 단백질 G로 정제된 액상 형태이며, 1 mg/mL 농도. 핵막 단백질 연구 및 세포핵 구조 분석에 적합.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3A3 |
| Immunogen | Amino acids 684–694 corresponding to the C-terminus of human LAP2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896822 |
Product Specific Information
Western blot detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).
Target Information
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes defining the border between the nucleus and cytoplasm. The inner membrane is lined by a filamentous meshwork called the nuclear lamina, composed of lamins. Lamins attach to the inner nuclear membrane through Lamin Associated Polypeptides (LAPs). LAP 1A, 1B, and 1C share high sequence homology, while LAP 2 is distinct. LAP 1A/B bind lamins A, C, and B1, whereas LAP 2 binds lamin B1 only. LAP 2 also binds to chromosomes, and its phosphorylation during mitosis inhibits these interactions, indicating its role in nuclear envelope reassembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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