Thermo Fisher Scientific GFP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:7500

View 182 publications 182 publications

Immunohistochemistry (IHC)

Assay-dependent

View 359 publications 359 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 15 publications 15 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 23 publications 23 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 13 publications 13 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 150 publications 150 publications

Flow Cytometry (Flow)

-

View 6 publications 6 publications

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 36 publications 36 publications

ChIP assay (ChIP)

-

View 12 publications 12 publications

in situ PLA (PLA)

-

View 2 publications 2 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Gel Shift (GS)

-

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 89 publications 89 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The GFP was isolated directly from the jellyfish Aequorea victoria.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.01% thimerosal

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_221570

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.