Thermo Fisher Scientific MEPCE Polyclonal Antibody
MEPCE 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간 및 랫트 반응성을 가지며, 고순도 정제 및 안정화 버퍼로 제공됩니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (KQQRKFQYGNYCK) corresponding to the internal amino acids of MEPCE (aa 408–420) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10985850 |
Product Specific Information
- This antibody is predicted to react with mouse based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:2,000.
Target Information
S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), stabilizing it (PubMed:17643375, PubMed:19906723, PubMed:30559425).
Also has a non-enzymatic function as part of the 7SK RNP complex, which sequesters the positive transcription elongation factor b (P-TEFb) in an inactive complex, preventing RNA polymerase II phosphorylation and transcriptional elongation (PubMed:17643375).
The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed:28254838).
In the 7SK RNP complex, MEPCE stabilizes 7SK RNA and facilitates complex assembly (PubMed:19906723).
Interaction with LARP7 within the 7SK RNP complex occludes its catalytic center (PubMed:19906723).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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