Thermo Fisher Scientific PRAS40 Monoclonal Antibody (73P21)
PRAS40 단백질을 인식하는 Thermo Fisher의 단클론 항체로, WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. 인간에 반응하며 정제된 액상 형태로 제공. Akt/mTOR 신호전달 연구에 유용. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 73P21 |
| Immunogen | Recombinant full length human PRAS40 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536321 |
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1 (PRAS40) is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
It is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and the cell cycle, and a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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