Thermo Fisher Scientific EIF2S1 Monoclonal Antibody (EIF2-alpha)
EIF2S1 단클론 항체로 인간, 마우스, 랫 등 다양한 종에 반응. Western blot, IHC, ICC, Flow, IP, RIP 등 다중 응용 가능. Recombinant EIF2-alpha를 면역원으로 사용하며 고순도 Protein A/G 정제. 연구용으로 스트레스 반응과 단백질 번역 조절 연구에 적합.
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Thermo Fisher Scientific EIF2S1 Monoclonal Antibody (EIF2-alpha)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | 19 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | — |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | 1 publication |
| Flow Cytometry (Flow) | 3–5 µg/1×10⁶ cells | — |
| Immunoprecipitation (IP) | Assay-dependent | 1 publication |
| RNA Immunoprecipitation (RIP) | Assay-dependent | — |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse, Non-human primate, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EIF2-alpha |
| Immunogen | Recombinant human eIF-2-alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536316 |
Product Specific Information
This antibody recognizes the alpha subunit of eukaryotic translation initiation factor 2 (EIF2-alpha).
Recommended positive controls include:
- Human: Jurkat, CEM, HeLa cells
- Mouse: 3T3L1 cells
- Rat: PC-12 cells
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of alpha, beta, and gamma subunits.
This factor facilitates the binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner, forming the 43S pre-initiation complex.
Phosphorylation of EIF2A by stress-activated kinases inhibits EIF2B activity, blocking guanine nucleotide exchange and regulating translation initiation under stress conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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